SCIENTIFIC STUDY

Five EM Frequencies Increase Osteoporsis Health (study summary)

STUDY INFO

TITLE
Electromagnetic field treatment increases purinergic receptor P2X7 expression and activates its downstream Akt/GSK3β/β-catenin axis in mesenchymal stem cells under osteogenic induction
AUTHOR(S)

Yingchi Zhang, Wenkai Li, Chaoxu Liu, Jiyuan Yan, Xuefeng Yuan, Wei Wang, Huaixi Wang, Hua Wu, and Yong Yang

IN VITRO HUMAN RESULTS
No
JOURNAL
Stem Cell Research and Therapy
DATE
December 21, 2019

RATIONALE

Millions of osteoporosis cases cause suffering and create expense. A better treatment will ease suffering and cost of having the disease.

Osteoporosis can include:

  • reduced bone mass
  • bone tissue micro-architectural degradation
  • bone fragility
  • higher fracture risk

Current treatments include dietary changes and pharmaceuticals.

Pharmaceuticals have multiple side effects and are expensive.

Patients often avoid compliance with their prescriptions.

Electromagnetic field (EMF) therapies are safe.

EMFs are already used to treat bone fractures, osteoarthritis, rheumatoid arthritis and non-union fractures.

EMFs have already been shown to positively affect bone stem cells, bone formation, and bone resorption (keeping the existing bone).

HYPOTHESIS

Stimulating P2X7 will stimulate bone marrow stem cells to decrease osteoporosis severity.

METHOD

Researchers studied both bone stem cells and ovaries.

Stem Cell Study

For the stem cell study, they extracted stem cell RNA from mice.

They placed the cells into an osteogenic (nutritional) medium.

They placed the enhanced cells into a C02 incubator.

Using coils, they applied EMFs to the RNA.

All samples were irradiated at 1 mT (micro tesla).

They applied 7.5 Hz, 15 Hz, 30 Hz, and 75 Hz to the cells in the osteogenic media.

Cells received stimulation for seven, 14, or 21 days.

After treatment, researchers measured the samples for P2X7 and for protein levels.

Ovary Study

Researchers placed EMF coils between cages.

Researchers placed rate into these cages.

The rates were divided into groups:

  • no EMF
  • EMF
  • EMF and BzATP (P2X7 stimulator)
  • inject with NaCl (sodium chloride)

Researchers exposed all groups to 15 Hz at 1 mT for 9 hours per day.

They removed the mouse ovaries at 8 weeks.

They euthanized the mice at 12 weeks.

Researchers also excised the right fimur and tibia of each mouse, and performed micro CT scans on these bones.

RESULTS

An application 15 Hz fields increased P2X7 expression compared to the control group.

Applying an osteogenic medium and stimulating it with 15 Hz fields was beneficial to treating osteoporosis.

The P2X7 upregulated in response to the fields.

The treatment was favorable to bone formation.

P2X7 expression increased at all frequencies and period. 15 Hz provided the optimal results.

CONCLUSION

EMF by itself was unsuccessful in increasing favorable osteoporosis reversal conditions

EMF in the presence of P2X7  increased “osteogenic marker expression” [the combination was therapeutic for osteoporosis]

EMF CHANNEL COMMENTARY

  • EMF combined with stem cells can heal osteoporosis
  • exposing stem cells to 15 Hz/1 mT creates conditions favorable for bone reformation
  • mouse ethical considerations seemed perfunctory

MORE INFO

  • EMF acts on rat bone marrow mesenchymal stem cells to promote differentiation to osteoblasts and to inhibit differentiation to adipocytes.
  • The effects of low-intensity pulsed ultrasound and pulsed electromagnetic fields bone growth stimulation in acute fractures: a systematic review and meta-analysis of randomized controlled trials.
  • The effect of pulsed electromagnetic fields in the treatment of osteoarthritis of the knee and cervical spine
  • Pulsed electromagnetic stimulation in nonunion of tibial diaphyseal fractures.
  • Treatment of nonunion using pulsed electromagnetic fields: a retrospective follow-up study